MEDINA develops different phenotypic and target-based assays, using enzymatic, protein-protein interactions, and whole cell approaches, both on-demand and optimized tests.
Custom development from your tube assay to a microtiter plate (96/384) assay. MEDINA has extensive experience in automation and miniaturization, selecting the most suitable screening strategy for your target.
High Throughput Screening Services
- Vector cloning: we subclone your gene of interest into our portfolio of expression vectors, covering a representative set of fusion constructs.
- Subsequent expression optimization of the resulting plasmids in a set of E. coli strains is then performed to maximize the amount of soluble, fully-folded, active protein.
- Established cell lines are generated with your wild-type or mutated gene of interest.
- Protein purification: scale-up of the optimal expression condition is followed by scouting of different lysis methods, chromatographic steps and a buffer formulation screen, to push the pure protein yield to the milligram level, so that it is ready to go for your biophysical experiments and your target-based screens.
- Detection tools for identifying hit compounds through biochemical, cell-based and microbial cell-based assays
- We perform phenotypic and target-based screening
- High capacity screening: hit discovery and hit-to-lead, assay data quality, 1 million data points per month.
- HTS assay formats: 96- and 384-wells plates.
- Automated laboratories: robotic equipment and liquid handling capacities from 2.5 nl to 1 ml.
- Customized assay development based on client specifications
- Screening Libraries: proprietary Natural Products libraries (see NP library Section), partners and commercial libraries
- Screening process including orthogonal and counter assays
- Stringent validation and statistical analysis, rule-based quality control system
- Absorbance
- Fluorescence: FI, FRET, TRF-FRET (HTRF®), FP, Bimolecular Complementation (BiMC)
- Luminiscence: glow, flash, dual luminescence, BRET, NanoBRET®
- AlphaScreen®, AlphaLISA®
- LC-MS/MS and LC/HRMS
- SelecT robot, automated cell culture system
- Liquid Handling Systems (from 1 µl to 1 ml) with 96- and 384-pipetting heads (Freedom EVO, Tecan; Biomek FX and i7, Beckman; EP3, PerkinElmer)
- Echo® Acoustic Liquid Handling System (from 15 nl to 1 µl), Beckman
- Multidrop Combi System (from 0.5 µl to 2.5 ml) for 96- and 384-well plates (ThermoFisher)
- Absorbance
- Fluorescence: FI, FRET, TRF-FRET (HTRF®), FP, Bimolecular Complementation (BiMC)
- Luminiscence: glow, flash, dual luminescence, BRET, NanoBRET®
- AlphaScreen®, AlphaLISA®
- LC-MS/MS and LC/HRMS
Quality Controls: We perform QC of each screening plate automatically on signal/background ratio and Z´ score calculations, considering the best plate layout for your assay; a statistically optimal number of repeats are performed for the internal positive and negative controls, and/or a dose-response curve of relevant validated inhibitors when available. Genedata Screener® software and in-house applications help us perform the analysis, visualizations, and management of screening data.
