HTS miniaturized assays based on microbial growth inhibition (cells, conidia and/or mycelia) using microtiter plates from 96-384 wells format. Development of taylor made inhibition assays for each wild type phytopathogen

• Pathogens of wheat and cereal crops:
  • Clavibacter michiganensis subsp. michiganensis (maize diseases)
  • Fusarium culmorum (common root rot and other cereal diseases)
  • Fusarium graminearum (devastating disease on wheat and barley)
  • Magnaporthe grisea (rice blast)
  • Zymoseptoria tritici (septoria leaf blotch on wheat crops)

• Pathogens of important dicot crops:
  • Botrytis cinerea (gray mold on vegetables, grape, strawberry crops)
  • Fusarium proliferatum (disease in asparagus, garlic, soybean crops)
  • Phytophthora infestans (pathogens of important dicot crop species)
  • Pseudomonas syringae (tomato and cruciferous diseases)
  • Xanthomonas campestris (black rot in cruciferous vegetables)

• Pathogens of important fruit and vegetable crops:
  • Colletotrichum acutatum (anthracnose of citrus and olive crops)
  • Fusarium cubense TR4 (wilt of banana)
  • Fusarium raphani (wilt of radish)
  • Monilinia laxa (causal agent of brown rot of stone fruit crops)
  • Rosellinia necatrix (cause of white root rot of several fruit crops)
  • Verticillium dahliae (verticillium in olive, tomato, potato crops)
  • Pseudomonas syringae (apical and leaves necrosis of mango and tomatoes)
  • Clavibacter michiganenesis (causal agent of necrosis of tomatoes plant)
  • Pectobacterium carotorum (causal agent of necrosis of onion and potato bulb)
  • Xanthomonas campestris (causal agent of necrosis of several plants)
  • Xylophilus ampelinus (causal agent of necrosis of vascular system of vine)

•  TECHNOLOGIES:
  • Absorbance
  • Fluorescence: fluorescence intensity (FI)
  • Imaging-halo Inhibition zone (Solid Agar Assays)

• LIQUID HANDLING SYSTEMS:
  • Liquid Handling Systems (from 1 µl to 1 ml) with 96- and 384-pipetting heads (Freedom EVO, Tecan; Biomek FX e i7, Beckman; EP3, PerkinElmer)
  • Echo® Acoustic Liquid Handling System (from 15 nl to 1 µl), Beckman
  • Multidrop Combi System (from 0.5 µl to 2.5 ml) for 96- and 384-well plates (ThermoFisher)

• READING SYSTEMS:
  • Multiplate reading system (Envision & Victor-2, PerkinElmer)
  • In-house Image Analyzer

Miniaturized agar based assays on microbial nutrient acquisition or siderophores production using microtiter plates 96 wells format.

• Solubilizing biofertilizers:
  • Phosphate solubilization: NBRIP assay and/or PIKOVSKAYA assay
  • Carbonate solubilization: MEA+CaCO3 assay

• Nitrogen fixing Biofertilizers:
  • Nitrogen fixation ability: GNFMM assay

• Compost Biofertilizers:
  • Cellulose, Pectin, Chitin solubilization and enzymatic assays

• Siderophores production:
  • CAS assay

• TECHNOLOGIES:
  • Fluorescence: fluorescence intensity (FI)
  • Imaging-halo Inhibition zone (Solid Agar Assays)

• LIQUID HANDLING SYSTEMS:
  • Liquid Handling Systems (from 1 µl to 1 ml) with 96- and 384-pipetting heads (Freedom EVO, Tecan; Biomek FX e i7, Beckman; EP3, PerkinElmer)
  • Multidrop Combi System (from 0.5 µl to 2.5 ml) for 96- and 384-well plates (ThermoFisher)

• READING SYSTEMS:
  • Multiplate reading system (Envision & Victor-2, PerkinElmer)
  • In-house Image Analyzer