MEDINA has a huge experience in the development of different biochemical assays, both on-demand and optimized tests. We can perform custom development from your tube assay to a microtiter plate (96/384) assay for High-Throughput Screening (HTS), hit confirmation, hit-to-lead and Mechanism of Action (MoA) of the lead.
Biochemical Assays
- Kinetics and endpoint measurements
- KINASES: GSK3-β, CDK1, CDK 2 & Casein I
- In vitro antioxidant assays: DPPH, ABTS, TEAC, ORAC
- Redox active compound determination: Resazurin-based assay, HRP-phenol red assay
- Enzyme-linked ImmunoSorbent Assays (ELISAs): interleukins, cell surface markers, ACE2/SARS-CoV2. Samples: conditioned cell medium, plasma, serum
- Enzymatic assays by LC-MS/MS and LC/HRMS
- On-demand assays:
- See Protein Lab Section for more information.
- Recombinant proteins: Cloning, protein-expression scouting and scale-up
- Expression profiling: RT-PCR, western blot
- In-gel technologies and assays: western-blot, SDS-Page, electromobility shift assays (EMSA)
- Thermofluor® (extrinsic fluorescence, Sypro Orange; intrinsic fluorescence coupled to fluorescent co-factors, FAD) applied to drug discovery, buffer formulation and the screening of protein variants
- Protein-protein interaction readouts (Homogeneous Time-Resolved, Fluorescence Polarization, competitive ELISA)
- Biophysical characterization of biologics (proteins, peptides, antibodies)
- Absorbance
- Fluorescence: FI, FRET, TRF-FRET (HTRF®), FP, Thermal Shift (Thermofluor®),
- Luminiscence: glow, flash, dual luminescence, BRET
- AlphaScreen®, AlphaLISA®
- LC-MS/MS; LC-HRMS
- Liquid Handling Systems (from 1 µl to 1 ml) with 96- and 384-pipetting heads (Freedom EVO, Tecan; Biomek FX e i7, Beckman; EP3, PerkinElmer)
- Echo® Acoustic Liquid Handling System (from 15 nl to 1 µl), Beckman
- Multidrop Combi System (from 0.5 µl to 2.5 ml) for 96- and 384-well plates (ThermoFisher)
- Multiplate reading system (Envision & Victor, PerkinElmer)
- CFX96/384 Real-Time PCR Detection System (Biorad)
- Mass spectrometry (Api4000 (Sciex) and QTOF5600 (Sciex))
- HPLC-DAD (Agilent 1290 DAD)
Quality control: We perform QC of each screening plate automatically on signal/background ratio and Z´ score calculations, considering the best plate layout for your assay; a statistically optimal number of repeats are performed for the internal positive and negative controls, and/or a dose-response curve of relevant pharmacological standards when available. Genedata Screener® software and in-house applications help us perform the analysis, visualizations, and management of screening data.
